Effects of nanoplastic exposure on the growth performance and molecular characterization of growth-associated genes in juvenile Macrobrachium nipponense

Abstract

Nanoplastic pollution has become a major issue in the aquatic environment while there are few studies examined the effects of nanoplastic exposure on crustaceans. To investigate this issue, we exposed juvenile shrimp, Macrobrachium nipponense to 75 nm polystyrene with 0, 5, 10, 20 and 40 mg/L nanoplastics concentrations for 28 days. The effects of nanoplastic exposure on the microstructure of the hepatopancreas, digestive enzyme activity and expression of growth-related genes were studied. The results showed that (1) adverse effects on the hepatopancreas were positively correlated with nanoplastic concentration; (David et al.) the activity of lipase, trypsin and pepsin was initially promoted and then inhibited with increasing nanoplastic concentration, whereas the activity of amylase was not significantly affected; (3) molting-associated genes were initially promoted and then inhibited with increasing nanoplastic concentration; (4) CDK2 gene was first cloned and molecular characteristics were analyzed. (5) polystyrene nanoplastics concentration >10 mg/L showed inhibition effect on CDK2 expression. These results indicated that nanoplastics affect the growth, digestive enzyme activity, hepatopancreas function and growth-related gene expression.Capsule: Our results identified the effects of nanoplastics on the growth performance of Macrobrachium nipponense in terms of digestion and molting.