Effects of Multi-enzyme Supplementation on Gut Morphology and Histomorphology in Broilers

Abstract

A biological trial in broilers was conducted with 7 treatments namely control (T1) as per nutrient requirements of poultry (NRC, 1994) specification, (T2) control plus 0.05 and (T3) 0.1% enzyme and control plus 0.05 and 0.1% enzyme, with (T4) 0.2%, (T5) 0.4% reduction in dicalcium phosphate with enzyme mixtures plus phytase with (T6) 2.5% and (T7) 5% reduction in metabolizable energy, crude protein, lysine and DL methionine with enzyme mixtures at 0.05 and 0.1% respectively. The villi height (μ) and villi width (μ) significantly (P<0.01) increased in enzyme treated groups (T2, T3, T4, T5&T7 and T2, T3, T4, T5, T6&T7 respectively) compared to that of control group. The number of crypts and goblet cells were decreased due to enzyme supplementation in T4, T5 and T7. The crypt height, crypt width and goblet cell height (μ) were significantly (P<0.01) reduced in all enzyme added groups than the control group. Similarly, the goblet cell width was reduced in T2, T4 and T5. No significant difference was noticed in intestinal weight (g/kg body wt.) and length (cm/kg body wt.) among the treatment groups.