Abstract
Objective To investigate the effects of MicroRNA-126 on glucose metabolism in the human liver cells. Methods The immortal liver-derived cell line Chang liver cell was taken as the object. Chang liver cells were transfected with MicroRNA-126 mimic, MicroRNA-126 inhibitor and relative negative control respectively, meanwhile the normal control group and Lipofectamine 2000 group were set too. The expression level of MicroRNA-126 was detected by reverse transcription-polymerase chain reaction (RT-PCR), and the protein expression of insulin receptor substrate-1(IRS-1), phosphatidylinositol 3-kinase p85β(PIK3R2), protein kinase B-2 (Akt- 2) and phosphorylated Akt- 2 (P- Akt- 2) were detected by using Western blotting. Data were analyzed with one-way ANOVA or t test. Results The expression of MicroRNA- 126 was significantly increased in MicroRNA- 126 mimic group compared with that in normal control group (799.70±66.46 vs 1.00±0.00, t=20.82, P 0.05). Compared with that in the normal control group, the P-Akt-2/Akt-2 decreased in MicroRNA-126 mimic group (0.53±0.07 vs 0.88±0.09, t=-5.298, P< 0.05). Conclusion MicroRNA-126 may affect glucose metabolism in human liver cells by inhibiting IRS-1/PIK3R2/Akt-2 signaling pathway. Key words: MicroRNA-126; Human liver cells; Glucose metabolism
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