Abstract

Administration of 10 and 30 μg methionine-enkephalin (MET-ENK)/g bw (n=10/dose) affected the propensity towards fighting in H. bimaculatus; 10 μg increased, while 30 μg decreased the aggressive behavior. MET-ENK also affected a number of behavior patterns displayed by the fish. Moreover, the “wet-dog-shakes” observed suggest that MET-ENK acts on opiate-receptors. Treatment with substance P (SP)/g bw (n=10/dose) induced chafing movements in the fish slightly. It also decreased fighting and increased biting of the air stone, which is evidence that H. bimaculatus is still aggressive, directing its attacks to different objects. When 4, 8, 12 μg somatostatin (SRIF)/g bw (n=10/dose) were injected, H. bimaculatus stopped fighting for several hours after the onset of treatment, depending on the dosage. Somatostatin reduces blood glucose concentration, causing a sudden stop of aggressive behavior. 0.04, 0.1, 0.6, 1.0 and 3.0 IU prolactin (PRL)/g bw (n=5/dose) eventually decreased fighting and affected a number of behavior patterns displayed by the fish.

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