Abstract
One-day-old broilers were randomly allocated to five treatment groups: basal diet and orally administered sterile saline (negative control, n-control); basal diet challenged with E. coli O78 (positive control, p-control); basal diet supplemented with 1×108 CFU/kg L. plantarum 15–1 and challenged with E. coli O78 (LP); basal diet supplemented with 5 g/kg fructooligosaccharides (FOS) and challenged with E. coli O78 (FOS); and basal diet supplemented with both L. plantarum 15–1 and FOS and challenged with E. coli O78 (LP+FOS). The broilers in the LP, FOS, and LP+FOS groups displayed a decrease of crypt depth at day 14 compared with the control groups. Furthermore, at days 14 and 21, the broilers in the LP group exhibited reduced serum levels of diamine oxidase (DAO) compared with the p-control group (p<0.05), and the broilers in the LP+FOS group showed increased serum concentrations of IgA and IgG relative to both control groups and decreased DAO levels compared with the p-control group (p<0.05). Moreover, the LP group displayed higher levels of acetic acid and total short-chain fatty acids (SCFAs) compared with the p-control group at day 14 (p<0.05), and the FOS group showed higher levels of valeric acid and total SCFAs at day 21 (p<0.05). The LP+FOS group also displayed a higher level of butyric acid at day 14 (p<0.05). In conclusion, dietary supplementation with FOS improved the growth performance, while supplementation with L. plantarum 15–1 and FOS improved intestinal health by increasing the levels of SCFAs and mitigating the damage caused by E. coli O78, thus preventing intestinal damage and enhancing the immune response.
Highlights
Escherichia coli-induced diarrhea has become a global public health problem in both developed and developing countries
The aim of this study was to investigate whether dietary supplementation with L. plantarum 15–1 and FOS alone or in combination reduces the negative effect on the intestinal morphology and the decline of the immune response induced by E. coli O78
Challenge with E. coli O78 lowered the average weight at day 21 and the average daily feed intake (ADFI) was decreased (p
Summary
Escherichia coli-induced diarrhea has become a global public health problem in both developed and developing countries. The prevention and treatment of this disease is predominantly based on vaccines and drugs. Science and Technology Department (2017NK2322, 2016NK2101, 2016WK2008, 2016TP2005) to SD. The authors acknowledge the academy of science and technology of state administration of grain for their support to the study
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