Abstract
Grafts of human skin on nude mice were subjected to a single dose of either 2 1/2 min or 4 min of radiation from two different commercial dental light curing units with emission mainly in the visible light spectrum but also with a small fraction of UV-A light. Seventy-two hours after exposure the tissue was examined for presence of Langerhans cells using monoclonal antibody OKT6 double layer immunofluorescence staining. Epithelial hyperplasia and reduced reactivities for OKT6 were seen after 2 1/2 min exposure. After 4 min of exposure OKT6 positive cells were completely absent from the epithelium. The results indicate that emission from dental light curing units can affect Langerhans cells in human epithelium and could thus modify the local immunologic response.
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