Abstract
The aim of this study was to examine the effects of immobilizing rhPDGF-BB plus rhBMP-2 on heparinized-Ti implants on in vivo osseointegration and vertical bone regeneration at alveolar ridges. Successful immobilizations of rhPDGF-BB and/or rhBMP-2 onto heparinized-Ti (Hepa/Ti) were confirmed by in vitro analysis, and both growth factors were found to be sustained release. To evaluate bone regeneration, rhPDGF-BB, and/or rhBMP-2-immobilized Hepa/Ti implants were inserted into beagle dogs; implant stability quotients (ISQ), bone mineral densities, bone volumes, osseointegration, and bone formation were assessed by micro CT and histometrically. In vivo study showed that the osseointegration and bone formation were greater in the rhPDGF-BB/rhBMP-2-immobilized Hepa/Ti group than in the rhPDGF-BB-immobilized Hepa/Ti group. The rhPDGF-BB/rhBMP-2 immobilized Hepa/Ti group also showed better implant stability and greater bone volume around defect areas and intra-thread bone density (ITBD) than the rhBMP-2-immobilized Hepa/Ti group. However, no significant differences were observed between these two groups. Through these results, we conclude rhBMP-2 immobilized, heparin-grafted implants appear to offer a suitable delivery system that enhances new bone formation in defect areas around implants. However, we failed to observe the synergetic effects for the rhBMP-2 and rhPDGF-BB combination.
Highlights
Dental implants have been generally used as credible and secure treatments for the restoration of function and aesthetics of edentulous patients [1]
Recombinant human platelet-derived growth factor-BB, recombinant human bone morphogenic protein-2, rhPDGF-BB, and recombinant human BMP-2 (rhBMP-2) enzyme-linked immunosorbent assay kit (ELISA) kits were purchased from PeproTech Inc. (Rocky Hill, NJ, USA)
Ti modified with rhHepa-DOPA, rhPDGF-BB, rhBMP-2, or rhPDGF-BB/rhBMP-2 had surface morphologies similar to Ti alone
Summary
Dental implants have been generally used as credible and secure treatments for the restoration of function and aesthetics of edentulous patients [1]. Patients who have insufficient bone quality and quantity, or poor healing and regenerative capacities have been reported to experience unfavorable results after implant treatment [2]. Several studies have reported that rhBMP-2 has no significant effect on bone formation [9,10]. These negative results were suggested to be due to large initial release of rhBMP-2, lack of standardization of the optimal rhBMP-2 concentration, and the use of only one type of growth factor, as natural regeneration process in man involves multiple growth factors [11,12,13,14]
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