Abstract
This study investigated the effects of IGF-I and EGF on the development of blastocysts or hatched blastocysts during the in vitro culture of embryos from immature porcine oocytes. After the in vitro maturation and fertilization of cumulus-oocyte complexes (COCs) and their culture in vitro in PZM3 medium, we examined the embryo development rate for 168 h. When different concentrations of IGF-I (0, 1, 10, 20 ng/ml) were supplemented to fertilized porcine embryos in vitro, there were no significant differences in cleavage rate, blastocyst development rate or blastocyst hatching rate among the treated groups. On the other hand, when different concentrations of EGF (0, 1, 10, 20 ng/ml) were supplemented to the in vitro culture medium, blastocyst development rate was highest in the group in which EGF was not supplemented and, specifically, it was higher than in the 20 ng/ml treatment group (p<0.05). When 10 ng/ml IGF-I and 1 ng/ml EGF were supplemented separately or simultaneously, there were no significant differences among the treated groups in blastocyst hatching rate and the number of cells in each condition. This study demonstrated that the addition of IGF-I and EGF into PZM3 medium did not enhance development of the blastocyst stage and total cell number in blastocysts. (Key Words : Embryo Development, Porcine Embryo, Growth Factor, PZM3)
Highlights
IntroductionThe in vitro production of porcine embryos has been an important basis for the study of the generation process of mammalian embryos; it is widely adopted for cutting-edge biotechnologies such as transgenetic animal cells, development of micromanipulation (Lee et al, 2007), and stem cell studies, etc
The in vitro production of porcine embryos has been an important basis for the study of the generation process of mammalian embryos; it is widely adopted for cutting-edge biotechnologies such as transgenetic animal cells, development of micromanipulation (Lee et al, 2007), and stem cell studies, etc.In general, the in vitro production of mammalian embryos is generally classified into maturation, fertilization and culture of embryos
Embryo development during insulin-like growth factor I (IGF-I) supplementation The in vitro fertilization rates were 66.9%, 75.8%, 68.8% and 76.7% for the treatment groups, and there were no significant differences among the groups
Summary
The in vitro production of porcine embryos has been an important basis for the study of the generation process of mammalian embryos; it is widely adopted for cutting-edge biotechnologies such as transgenetic animal cells, development of micromanipulation (Lee et al, 2007), and stem cell studies, etc. The in vitro production of mammalian embryos is generally classified into maturation, fertilization and culture of embryos. In the culture of porcine embryos Whitten's medium, Beltsvile embryo culture medium (BECM-3), North Carolina State University (NCSU-23) and Porcine zygote Medium 3 (PZM3) are used. PZM3 is most commonly used and has recently resulted in better outcomes than any other medium (Yoshioka et al, 2002). The culture medium for embryos is composed of simple minerals, energy sources, amino acids, pH buffers, trace elements and antibiotics.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
