Abstract
Aim:The objective of this research was to study and to prove the effectiveness of hyperbaric oxygen therapy (HBOT) starting time on liver morphofunctional changes after ischemia-reperfusion in the hind limb of rabbits.Materials and Methods:This research used a complete randomized design with 4 groups and 6 repetitions on each. After 6 h artery femoral is ligation, reperfusion was performed for 100 min (G1), HBOT for 90 min after 10 min reperfusion (G2), 10 min reperfusion (G3), and HBOT 90 min after 60 min reperfusion (G4). Then, all of the rabbits were sacrificed. The liver and blood were taken for histopathological changes examination as well as for measuring the level of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The statistical test using Kruskal–Wallis and Mann–Whitney showed that the score of degeneration, necrosis, and portal inflammation in groups without HBOT (G1 and G3) were not significantly different, as well as in group with HBOT (G2 and G4) (p>0.05). However, the scores of histopathological changes in G1 and G3 were significantly different from those in G2 and G4 (p<0.05). The levels of AST and ALT in the groups without hyperbaric therapy (G1 and G3) were not significantly different from those in the groups treated with hyperbaric therapy (G2 and G4) (p>0.05).Result::Hind limb ischemia injury reperfusion can trigger damage for liver morphology, but not lead to liver dysfunction. Reperfusion can trigger increased activity of neutrophils, while neutrophil infiltration in the organ will lead to dysfunction. HBOT can inhibit the activity of neutrophils and the dysfunction of organs caused by ischemic reperfusion.Conclusion:HBOT for 90 min, both 10 and 60 min after the reperfusion, can protect hepatocytes from damage.
Highlights
Ischemic condition can occur in a wide variety of disease status
hyperbaric oxygen therapy (HBOT) can inhibit the activity of neutrophils and the dysfunction of organs caused by ischemic reperfusion
The results showed that within groups without hyperbaric therapy (G1 and Group 3 (G3)), the scores of necrosis, degeneration, and portal inflammation were not significantly different (p>0.05)
Summary
An ischemic condition often occurs in a variety of surgical procedures such as in a surgical process of vascular or organ transplantation. In such circumstances, ischemic injury can occur when blood flow to a tissue is reduced or stopped in a long time, resulting changes in the metabolism of muscles, from aerobic state into anaerobic one. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
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