Effects of gelatin on proliferation of cord blood derived mesenchymal stem cells

Abstract

Mesenchymal stem cells (MSCs) are isolated from various tissues including bone marrow (BM), placenta, adipose tissue, and umbilical cord blood (CB). Compared to MSC isolated from BM (BM‐MSC), MSC from cord blood (CB‐MSC) has a short logarithmic growth stage before entering the senescence stage in vitro long‐term culture. Gelatin coated dishes are used to culture for embryonic stem cells, primary cell and clones. To determine whether the usage of gelatin‐coated dishes gave superior effects on efficient isolation MSC from CB and longer proliferation of CB‐MSC in vitro, we cultured mononuclear cells (MNC) isolated from human CB with gelatin‐coated or uncoated dishes. In the results, CB‐MSC in gelatin‐coated dishes revealed higher and longer proliferation than those in uncoated dishes. To unveil the mechanism, we compared gene expression profiles of MSC cultured in gelatin‐coated and uncoated dishes using the microarray technology. Many of genes involved in adhesion molecule induced signaling pathway were identified. Our findings suggest that gelatin‐derived signaling may contribute to the expended proliferation and maintenance of CB‐MSC and confer the chance to study what signals involved to CB‐MSC proliferation.