Effects of Fullerene C60 and Fruit Extract of Balanites aegyptiaca Plant on the Nephrotoxicity and Hematotoxicity Induced by Doxorubicin in Diabetic Male Albino Rats.

Abstract

There was a relationship between hyperglycemia and pharmakinetic of toxicity of doxorubicin that leads to increasing oxidative stress on cells. This study was aimed to detect the curative effect of some antioxidants drugs (fullerene C60 and mesocarp fruit extract of Balanites aegyptaica) on the hematological and biochemical of kidney function parameters after induced by diabetes and toxicity with doxorubicin on the rats. nine groups of adult male albino rats were established (n= 8). The first group (Gp. 1) was served as a normal group. Gp. 2 was injected intraperitoneal (i.p.) with by streptozotocin at a single dose (45 mg/kg body weight). Diabetic rats (Gp. 3)were injected intraperitoneal (i.p.) with doxorubicin at the dose (5mg/kg body weight)for three days.Diabetic rats (Gp. 4) was given orally fullerene C60 daily at the dose (4 mg/kg body weight).Diabetic rats (Gp. 5) treated orally bymesocarp fruit extract of Balanites aegyptaicaat dose (1.5ml/kg. body weight). Diabetic rats(Gp. 6) treated orally with fullerene C60 plus mesocarp fruit extract of Balanites aegyptaica. Diabetic rats (Gp. 7) intoxicated DOX treated orally with fullerene C60. Diabetic rats (Gp. 8) intoxicated DOX treated orally with mesocarp fruit extract of Balanites aegyptaica. Diabetic rats (Gp. 9) treated orally with fullerene C60 plus mesocarp fruit extract of Balanites aegyptaica. Whole blood and serum were collected for hematological and biochemical of kidney functions examinations, respectively. Diabetes induces, hematotoxicity was determined by a highly significant decrease in red blood cells (RBCs), white blood cells (WBCs), blood Platelets (PLTs), haemoglobin (Hb) and PCV value, as well as nephropathy was determined by a highly significant increase in creatinne, urea and uric acid. diabetic rats intoxicated DOX showed the reduction in hematological parameters, also, there were a highly significant increase in kidney function parameters. Fullerene C60 and mesocarp fruit extract of Balanites aegyptaica meliorated hematological and kidney functions indices. It could be concluded that Fullerene C60 and Balanites aegyptiaca, clarified a modulatory role against the cellular damage produced by oxidative stress.