Effects of Divalent Cations on Outward Potassium Currents in Leech Retzius Nerve Cells

Abstract

AbstractThe present study examines the effects of divalent metals, cadmium (Cd2+) and manganese (Mn2+), on the outward potassium currents of Retzius cells in the hirudinid leeches Haemopis sanguisuga using conventional two-microelectrode voltageclamp techniques. The outward potassium current is activated by depolarization and plays an important role in determining both the neuronal excitability and action potential duration. A strong inhibition of the fast current and a clear reduction in the late currents of the outward current with 1 mM Cd2+were obtained, which indicated that both components are sensitive to this metal. Complete blockage of the fast and partial reduction of the slow outward currents was observed after adding 1 mM Mn2+to the extracellular fluid. These data show that the outward K+current in leech Retzius nerve cells comprises at least two components: a voltage-dependent K+current and a Ca2+- activated K+current. These observations also indicate that Cd2+is more eff ective than Mn2+in blocking ion fl ow through these channels and that suppressing Ca2+-activated K+outward currents can prolong the action potential in nerve cells.