Abstract
The genus Ziziphora of the family Lamiaceae is well-known for medicinal properties of its species. Ziziphora persica is an edible medicinal plant, which is wildly distributed in Iran and is famed as a wild vegetable with notable aroma and flavor. We investigated the effects of different strengths of Murashige and Skoog medium (Full MS, 1/2 MS, 1/3 MS, 1/4 MS) on the production of phenolic and flavonoid compounds in cultured nodal segments of Z. persica. Although the highest dry and fresh weights as well as highest amounts of pigments were obtained in full MS medium, the maximum mean shoot number and shoot length were achieved using 1/2 MS medium. Intriguingly, the highest amount of phenolics (27.41 ± 4.82 mg PYRO/g DW) and flavonoids (352.80 ± 5.80 mg QE/g DW) were observed on quarter strength medium. Therefore, MS medium with higher strength was appropriate for growth and MS medium with lower concentration was found more suitable for production of phenolics and flavonoids.
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