Effects of dietary terrestrial oils supplemented with L-carnitine on growth, antioxidant capacity, lipid metabolism and inflammation in large yellow croaker (Larimichthys crocea).

Abstract

The present study was conducted to determine the effects of dietary terrestrial oils (TO) supplemented with L-carnitine on growth performance, biochemical and antioxidant response, lipid metabolism and inflammation in large yellow croaker (Larimichthys crocea). Three iso-nitrogenous and iso-lipidic experimental diets were formulated with FO (fish oil, the control group), 75% TO (75% FO was substituted by the oil mixture with equal amounts of soybean oil, linseed oil and pork lard) and 75% TOC (75% TO supplemented with 800 mg/kg L-carnitine), respectively. Compared to the control group, feed efficiency ratio and specific growth rate were significantly increased in fish fed diets with 75% TO and 75% TOC. Hepatic lipid content, serum triglyceride level, low-density lipoprotein-cholesterol level and the mRNA expression of pro-inflammatory genes (tnfα and ifnγ) were significantly increased in fish fed the diet with 75% TO compared to the control group. However, the supplementation of 800 mg/kg L-carnitine in the 75% TO diet repressed hepatic lipid content, serum low-density lipoprotein-cholesterol level and the mRNA expression of tnfα and ifnγ in fish compared to fish fed the diet with 75% TO. Total antioxidant capacity, the activity of superoxide dismutase, the mRNA expression of cpt-I and the activity of CPT-I were significantly increased in fish fed the diet with 75% TOC compared to 75% TO. In conclusion, these results suggested that the supplementation of 800 mg/kg L-carnitine in the diet with terrestrial oils mixture could increase growth, antioxidant capacity, fatty acid oxidation and decrease the expression of inflammatory genes in large yellow croaker.