Abstract
The present study was conducted to determine the effects of Cnidium oficinale Makino (CO) extract on adipocyte differentiation, and to explore its mode of action in adipocyte differentiation. Post‐confluent mouse 3T3‐L1 cells were treated with differentiation medium containing 0, 50, 100, 200, and 400 ppm of CO methanol extract. The extent of adipocyte differentiation was evaluated by Oil Red O staining and measurement of glycerol‐3‐phosphate dehydrogenase (GPDH) activity. The IRS‐1, GLUT4, PPARγ and C/EBPα mRNA expressions were measured using RT‐PCR and protein expression by Western Blot. The results of Oil Red O staining and GPDH activity analyses showed that CO extract stimulated 3T3‐L1 adipocyte differentiation dose‐dependently up to 200 ppm, but reversed the effect at 400ppm. It has been shown that the CO extract may induce adipocyte differentiation through stimulation of PPARγ and C/EBPα expression. The CO extract also exhibited insulin‐like activity by inducing IRS‐1 and GLUT4 expressions. The results of the present study indicated bi‐potential effects of CO on adipogenesis, a plausible candidate for regulating obesity. The CO extract also showed some potential for regulating blood glucose level.
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