Abstract
The present study set out to investigate the effects of cigarette smoke on oxidant–antioxidant systems of homogenized bovine lung tissue and the possible protective effects of cigarette filters impregnated with vitamin C and lemon juice. Cigarette smoke was passed into bovine lung homogenate supernatant and malondialdehyde, superoxide dismutase, glutathione peroxidase, catalase, paraoxonase-1, nitric oxide synthase, and nitric oxide were determined. Malondialdehyde and nitric oxide levels were found to have increased as the dose of cigarette smoke increased and superoxide dismutase activity decreased with incremental doses of cigarette smoke exposure. Furthermore, the lemon juice impregnated filters scavenged nitric oxide. The cigarette smoke led to lipid peroxidation and a reduction in superoxide dismutase activity. Antioxidant-impregnated filters could not prevent oxidative stress.
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