Abstract
The binding of cationic ionenes onto budding protoplast was investigated and the results were associated with the cell viability. There are critical numbers of carbon atoms to induce effective cell disruption and cell binding. The longer the alkyl chain of the ionene, the lower the concentration at which cell disruption occurs. The ionenes with increased charge density undergo effective binding, while almost 2 orders of magnitude higher concentration are required for effective cell disruption. These results were associated with the cooperativity of the binding process, which induces local stress and solubilization of the lipid membrane.
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