Effects of cell deformability and adhesion strength on dynamic cell seeding: Cell-scale investigation via mesoscopic modeling

Abstract

The flow of cell suspension through a porous scaffold is a common process in dynamic cell seeding, which determines the initial distribution of cells for constructing tissue-engineered grafts. Physical insights into the transport and adhesion behaviors of cells in this process are of great significance to the precise control of cell density and its distribution in the scaffold. Revealing of dynamic mechanisms underlying these cell behaviors through experiments is still difficult. The numerical approach therefore plays an important role in such studies. However, existing studies have mostly focused on external factors (e.g., flow conditions and scaffold architecture) but ignored the intrinsic biomechanical properties of cells as well as their associated effects. The present work utilized a well-established mesoscopic model to simulate the dynamic cell seeding within a porous scaffold, based on which a thorough investigation of the effects of cell deformability and cell-scaffold adhesion strength on the seeding process was carried out. The results show that the increase in either the stiffness or the bond strength of cells would augment the firm-adhesion rate and thus enhance seeding efficiency. In comparison to cell deformability, bond strength seems to play a more dominant role. Especially in the cases with weak bond strength, remarkable losses of seeding efficiency and distribution uniformity are observed. Noteworthily, it is found that both the firm-adhesion rate and the seeding efficiency are quantiatively related to the adhesion strength which is measured as the detachment force, suggesting a straightforward way to estimate the seeding outcome.