Effects of bone marrow mesenchymal stem cells on blood glucose concentration in diabetic rats

Abstract

Objective To investigate the effects of different concentrations of bone marrow mesenchymal stem cells (BMSCs) on blood glucose concentration in diabetic rats. Methods SD rats’ bone marrow was isolated, purified and cultured to obtain BMSCs. The expression of surface markers CD34, CD44, CD45 and CD90 was detected. The models of diabetic rats were randomly divided into control group, the low concentration group, high concentration group and normal feeding group. Low concentration group and high concentration group were injected with 1×105 and 5×106 BMSCs respectively, and the normal group and the control group were injected with equal volume of phosphate buffer (PBS). The dynamic changes of blood glucose concentrations at 1st, 2nd and 3rd week after injection were statistically analyzed. Results BMSCs were proved by immunofluorescence with CD34 (-), CD44 (+ ), CD45 (-) and CD90 (+ ). After treatment with BMSCs, blood glucose had no significant change in the first two weeks, but decreased by 16.1% at 3rd week in the low concentration group. As compared with the control group, blood glucose concentration was significantly decreased, and decreased by 42.0% at 3rd week in the high concentration group. There was no obvious change in blood glucose concentration before and after treatment in the normal and control groups. Conclusion High concentration (5×106 cells) BMSCs can control and regulate the blood glucose concentration in diabetic rats. Key words: Bone marrow mesenchymal stem cells; Diabets; Rat; Blood glucose concentration