Abstract
To investigate the changes induced by DisBa-01 on repair of wound healing after induced incisional hernia (IH) in rats. Thirty two male albino rats were submitted to IH and divided into four experimental groups: G1, placebo control; G2, DisBa-01-treated; G3, anti-αvβ3 antibodies-treated and G4, anti-α2 antibodies-treated. Histological, biochemical and extracellular matrix remodeling analysis of abdominal wall were evaluated. After 14 days, 100% of the G2 did not present hernia, and the hernia ring was closed by a thin membrane. In contrast, all groups maintained incisional hernia. DisBa-01 also increased the number macrophages and fibroblasts and induced the formation of new vessels. Additionally, MMP-2 was strongly activated only in G2 (p<0.05). Anti- αvβ3-integrin antibodies produced similar results than DisBa-01 but not anti-α2 integrin blocking antibodies. DisBa-01 has an important role in the control of wound healing and the blocking of this integrin may be an interesting therapeutically strategy in incisional hernia.
Highlights
Incisional hernias (IH) are usually found as a complication of about 11% of abdominal wall closures
DisBa-01 has an important role in the control of wound healing and the blocking of this integrin may be an interesting therapeutically strategy in incisional hernia
Β3-integrin deficient mice showed accelerated re-epithelialization associated with enhanced TGF-β1 signaling[18]. These results suggest that the αvβ[3] integrin controls the rate of wound repair, and, it could be a target for new wound healing therapies
Summary
Incisional hernias (IH) are usually found as a complication of about 11% of abdominal wall closures. The distinct phases of wound healing process are well described and include local hemorrhage with extravasation of platelets and platelet-derived growth factors such as PDGF (platelet derived growth factor) and EGF (epidermal growth factor). These mitogens stimulate FGF-7 (fibroblast growth factor-7) expression by fibroblasts. Invading neutrophils and macrophages will secrete several proinflammatory cytokines and growth factors resulting in angiogenesis and fibroplasia. These events will culminate with the synthesis of a provisional matrix that must be able to support the biomechanical forces of the abdominal wall[2]
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