Abstract
The paper reports on a study of the effects of different type antioxidant molecules on recombinant, luminescent, bacterial biosensensors. It has been demonstrated that thiamine can activate oxidative stress promoters, mainly in biosensors based on the parental cells of Salmonella typhimurium LT2. We have detected the induction of soxS and katG promoters and activation of the SOS-response system in the analyzed biosensors in the presence of pyridoxine. It has been shown that ascorbic acid has pronounced prooxidant and toxic properties. Other analyzed compounds proved to be less active and did not induce antioxidant and reparatory systems in luminescent biosensors based on the parental cells of Salmonella typhimurium LT2 and Escherichia coli MG1655.
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