Effects of acute temperature increases on House sparrow (Passer domesticus) pectoralis muscle myonuclear domain.

Abstract

With rapid climate change, heat wave episodes have become more intense and more frequent. This poses a significant threat to animals, and forces them to manage these physiologically challenging conditions by adapting and/or moving. As an invasive species with a large niche breadth, House sparrows (Passer domesticus) exhibit high phenotypic flexibility that caters to seasonal changes in function and metabolism. For example, their pectoral muscle complex exhibits size and mass plasticity with winter and summer acclimation. Here, we investigated the effects of acute whole-organism heat stress to 43°C on cellular-level changes in House sparrow pectoralis muscle myonuclear domain (MND), the volumetric portion each nucleus is responsible for, that have gone overlooked in the current literature. House sparrows were separated into a control group, a heat-shocked group subjected to thermal stress at 43°C for 24 h, and a recovery group that was returned to room temperature for 24 h after experiencing the same temperature treatment. Here, we found that heat-shocked and recovery groups demonstrated a decrease in number of nuclei per millimeter of fiber and increase in MND, when compared with the control. We also found a significant positive correlation between fiber diameter and MND in the recovery group, suggesting the possibility that nuclei number constrains the extent of muscle fiber size. Together, these results show that acute heat shock alters House sparrow pectoralis muscle cellular physiology in a rigid way that could prove detrimental to long-term muscle integrity and performance.