Abstract
The effect of in vivo ACTH injection on adrenal cytosolic protein synthesis has been studied by two dimensional polyacrylamide gel electrophoresis. Using this sensible technique about 70 proteins were identified and the pattern found was much more complex than previously thought using monodimensional gel electrophoresis. These proteins were analyzed, and fell into 4 categories: the first group is composed of two proteins which are dramatically increased after 4 h of ACTH in vivo treatment. The second group is composed of 10 proteins whose stimulation by the hormone, although less dramatic, is still very important. In the third group (8 proteins) the stimulation is more discrete but constant and significant. The rest of the proteins are not affected by the hormonal treatment.
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