Abstract
An experiment was performed to assess the effects of starch infusion on the nutrient digestibility, mRNA abundance of gluconeogenesis and lipid metabolism in mid-lactating cows. Four multiparous Holstein cows fitted with permanent ruminal cannulas were arranged into a complete 4×4 Latin squares, abomasally infused starch solution at 0, 800, 1600, and 2400g/d for 15 days. Compared with control, starch digestibility was improved by starch infusion of 1600g/d (P<0.05). No difference was detected in body weight and BCS (P>0.05). Liver PEPCK-C mRNA abundance in 1600g/d starch infusion was significantly (P<0.05) higher than control and 2400g/d starch group. GLUT-4mRNA (P=0.12) tended to increase quadratically; the maximum abundance was in 800g/d infusion group; however, the minimum PPAR-gamma abundance was in 1600g/d starch infusion group (P=0.10). Overall, abomasally supplementation of different degrees of starch to mid-lactating cows could certainly be carried down to the intestine directly to ameliorate energy supplies, and therefore, transport to the subcutaneous adipose stored as lipid.
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