Abstract
To verify the effect of highly concentrated platelet-rich plasma (hPRP) in the pathways of bone repair using non-critical defects in the calvaria of rabbits. The hPRP was produced from collected venous blood of 21 rabbits. Four non-critical defects of 8 mm in diameter were created on the calvaria of each animal. The defects were all treated differently: autogenous particled bone (APB, group 1), autogenous particled bone associated with hPRP (APB + hPRP, group 2), isolated hPRP (group 3), and blood clot (control, group 4). Animals were submitted to euthanasia on the 2nd, 4th and 6th week postoperatively. Histological and histomorphometric analysis were carried through. After two weeks, groups 1 and 2 were in more advanced stage of repair than 3 and 4. At this period, comparing the groups 1 and 2, no significant differences were found between both, which also happened between the groups 3 and 4. However, after four and six weeks, the group 1 showed a more advanced stage of repair among all the other studied groups, while group 2 was in more advanced signs of bone repair than groups 3 and 4. Comparing groups 3 and 4, after four and six weeks, the least advanced stage of bone repair was found to be within group 3. The use of a highly concentrated PRP was considered prejudicial to the repair of non-critical defects in the calvaria of rabbits, either in the association of autogenous particled bone, when compared to autogenous particled bone alone, or in its isolated form, when compared to blood clot (control).
Highlights
Multiple uncertainties still exist about the action of the growth factors and the platelet-rich plasma and its effect on the tissue repair
The use of a highly concentrated platelet-rich plasma (PRP) was considered prejudicial to the repair of noncritical defects in the calvaria of rabbits, either in the association of autogenous particled bone, when compared to autogenous particled bone alone, or in its isolated form, when compared to blood clot
The present study aims to analyse the effects of a highly concentrated PRP on the mechanism of tissue repair of non-crictical defects in the calvaria of rabbits
Summary
Multiple uncertainties still exist about the action of the growth factors and the platelet-rich plasma and its effect on the tissue repair. It is known that platelets are a source of several growth factors, among which are; PDGF1-4, TGF-b5,6, VEGF7,8, IGF-I4,9 and EGF6,10. This fact stimulated the development of a platelet concentrate with the intention of increasing the levels of local growth factors delivery, which, theoretically, if present at a damaged site, could improve the healing process. There is still no consensus about the ideal platelet concentration that could optimize the tissue repair process, some in vivo and in vitro studies suggest that a PRP highly concentrated could even be harmful to the repair[13,14,15]. The present study aims to analyse the effects of a highly concentrated PRP (hPRP) on the mechanism of tissue repair of non-crictical defects in the calvaria of rabbits
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