Abstract
BackgroundThe study investigated the effects and mechanism of duodenal-jejunal bypass (DJB) and sleeve gastrectomy (SG) on the expression of liver GLUT2 and glucokinase (GCK) in diabetic rats.MethodsAnimal models of Goto–Kakizaki (GK) rats were established for the investigation of DJB and SG. Results of weight, food intake, fasting plasma glucose level, oral glucose tolerance test and insulin were compared. Liver tissues were harvested 8 weeks postoperatively. Reverse transcription-PCR and western blot were used to detect liver GLUT2 and GCK mRNA and protein expression after operation.ResultsFasting plasma glucose levels of DJB group and SG group in GK rats were markedly declined at 3 days and l, 2, 4, 6, and 8 weeks postoperatively (P <0.01), whereas the levels of the sham-operated group only dropped at 3 days and 1 week postoperatively, and there were no significant differences 2 weeks postoperatively (P >0.05). In the liver of GK rats, GLUT2 mRNA level and protein expression after DJB were higher than those in sham-operated group and control group. GLUT2 mRNA level and protein expression after SG were significantly lower than those in control group (P <0.01). GCK mRNA and protein experienced similar expression change.ConclusionsBoth DJB and SG can decrease the plasma glucose levels of GK rats, whereas they have different effects on the expression of liver GLUT2 and GCK.
Highlights
The study investigated the effects and mechanism of duodenal-jejunal bypass (DJB) and sleeve gastrectomy (SG) on the expression of liver GLUT2 and glucokinase (GCK) in diabetic rats
We aim to explore the effects of the two surgical methods on liver glucose transport protein 2 and liver glucokinase (GCK) expression to understand the role of surgery in glucose metabolism, and to study the mechanism of surgical treatment of diabetes
Fasting plasma glucose level Fasting plasma glucose levels of DJB group and SG group in GK rats were markedly declined at 3 days and l, 2, 4, 6, and 8 weeks postoperatively (P 0.05) (Figure 3)
Summary
The study investigated the effects and mechanism of duodenal-jejunal bypass (DJB) and sleeve gastrectomy (SG) on the expression of liver GLUT2 and glucokinase (GCK) in diabetic rats. Drugs can control blood glucose level, they rarely make patients’ blood glucose level become normal and do not prevent diabetes complications. Strict diet and repeated fluctuations in blood glucose levels place a continuous mental stress on patients and affects their quality of life. Investigators had found that many type 2 diabetes patients achieved clinical resolution after bariatric surgery of morbid obesity, which suggested that the diabetes may be cured by surgery. It was found that 95% diabetes patients with normal body mass index have controlled blood glucose after surgery [4]. Many studies suggest that hormonal effect resulting from the anatomical changes in the gastrointestine is the main mechanism of long-term stability of controlling blood glucose. There are studies that consider the high metabolic status and low calorie intake to be the major factors in controlling plasma glucose [5]
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