Abstract
Deslorelin is a synthetic GnRH analogue, which is being used as a contraceptive in animals by acting as a gonadal suppressant. The product Suprelorin (Virbac, Australia) contains deslorelin as a biocompatible, slow release subcutaneous implant. The continuous release of deslorelin provokes a down-regulation of GnRH receptors, and subsequently, inhibition of the synthesis and release of the gonadotropins FSH and LH, necessary for gonadal activities. The intention of this study was to investigate the effectiveness of a subcutaneous deslorelin acetate implant (2,35mg) in suppressing ovarian cyclic activity and inhibiting ovulation in captive Common Marmoset Callithrix jacchus, and investigate the reversibility of the treatment. Two experimental groups were formed, group deslorelin (D) with three couples and control group (C) with two couples. To monitor the effect of the implants, hormones indicating ovarian cyclic activity were monitored non-invasively by enzyme immunoassay (fecal monoclonal antibody anti-progesterone CL 425). Fecal samples were collected three times a week from all females during three trial phases (phase I: month 1,2,3 and 4; phase II: month: 5,6 and 7 and phase III: month 8,9 and 10). In contrast to expectations the results of this trial indicated that there was no suppression of the ovarian cyclic activity, nor inhibition of the ovulation after the application of the implants. The outcome of our trial can possibly be explained by the fact that the dosage of 2.35mg of deslorelin is not effective in C. jacchus. We confirmed significant changes (p<0.05) of P4 metabolites from phase I to phase II due to the treatment after the implantation of the GnRH analogue Deslorelin. The employed non-invasive fecal progesterone monitoring could be biologically validated and proved to be efficient in the detection of ovarian cyclic activity in this neotropical primate species, C. jacchus.
Highlights
In phase II, after the deslorelin implantation, all group D females showed promptly an initial increase in ovarian activity and concentrations of fecal progesterone metabolites increase above the peak values previously established for all females
Progesterone concentrations remained elevated during phase II and ovarian cyclic activity never seized
The results suggest that the concentration of 2.35mg deslorelin acetate was not effective to suppress ovarian cyclic activity in Callithrix jacchus, the deslorelin implants did
Summary
Toconfrtoflwtfildantimaflpopufaltionstincaptvitfiyttsi an timporfatnft managemenftaspecf,tastin mosftcasesspace andresourcesareftlimtfietdandsomespecteishaveahtgih reproductvierafet. Theentriesftudypertiodwasdtvitidedtinfthreephases: Tomtintimtziesfrtessanddtsicomfforf,t whtcihfforexampfel Phase One- Thtsi phaseatimedftocontri mfthe cofflelctingbfloodsampfels woufldcause, weempfloyed cycftlci ovartainactvitfiyt offaffllfef mafels by montfitorting a non-tinvastvie mefthodfto montfitor endocrtinoflogtciafl progesfetrone mefatboftlfiet felvefsl.Iftfalsfetdffour monfths changes(Munro&Sfatbenfeffldft1984).Thepresenftsftudy wasdestginedftotinvestgiafet wheftherasflowrefelaseoff fthe GnRH-agontsift woufldefefctviefyl suppressovartain cycftlci actvitfiyt tinaduffltfef mafelsoffC.jacchus,andfto demonsfrtafet fthereverstibtftilfiyt offfthe confrtaceptvie efefcftafetrtimpfalnftremovaf.l Inaddtitionfthesftudy atimedftovertfiyf fthesefelcfetd mefthodoffnon-tinvastvie montfitortingoffovartaincycftlci actvitfiyt bymeasurtingfefcafl progesfetrone mefatboftlfiet concenfrtations. Fecal hormone level after withdrawal of deslorelin The duration of this phase was three months. Samples were thawed and transferred to Eppendorf plastic tubes, prior marked with the animal and sample identification numbers They were placed in a vacuum freeze-dryer system where they were lyophilized at a temperature of -510C for 24 hours, thereby removing all moisture, preventing further deterioration of the fecal progesterone metabolites. The results of the concentrate obtained were expressed in ng/well
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
