Effectiveness phytochemistry test of aquatic plant Murdannia sp. in invitro for fish pathogen disease

Abstract

Fish disease is a major factor causing yield losses in fish farming production. The use of antibiotics that treat against diseases can pose the highest risk for the ecosystem and its overuse could cause the side effect, such as antibiotics-resistant diseases. Environmentally friendly production method of fish is thus needed. The objective of this study was to determine the active pharmacological substances and anti-bacterial activities extracted from Murdannia sp. as a potential herbal agent as an anti-bacterial treat fish diseases. The study was conducted from October 2017 to May 2018. Murdannia sp. aquatic plants were collected from the Bantimurung river waters Maros district, South Sulawesi. Phytochemical analysis was carried out at Laboratory of Pharmacology, University of Indonesia. Anti-bacterial analysis was carried out using the disc diffusion method in the KKP Fisheries Development Research and Management (IP4I), Depok. The results showed that an application of Murdannia sp. crude extract treated at dose of 0.1 g did not inhibit the growth of the bacterial pathogen of Aeromonas hydrophilla, Edwardsiella ictaluri, Streptococcus agalactiae, Flavobacterium columnare. However, at 0.2 g of Murdannia sp. extract level, the growth of Edwardsiella ictaluri was inhibited in intermediate inhibiton category which inhibition zone of 11 mm. Based on the ppharmacological test, it was known that Murdannia sp. aquatic plants consisted of three majority compound such as 0.084/10 g of flavonoid, 0.0558/10 g of Phenol, and 2/10 g of antioxidant 3036. These findings could be a basic research information in attempting to use aquatic plants as an alternative way for treating anti-bacterial fish disease in future.