Abstract
<p class="abstrak2">Recent technological developments to produce germ line chimeras with primordial germ cell (PGC) transfer into the recipient embryo provide an opportunity to conserve and retrieval of chicken genetic resources in complete form. The study was conducted to obtain the most effective DMSO percentage to recovery rate and viability of Gaok chicken PGC after freezing which will later be feasible to be transferred. In this study, the eggs of Gaok chicken were incubated for about 2.5 - 3 days to obtain embryos at stages 14 - 16. Blood retrieval was done through the dorsal aorta using micropipettes under microscope. The procedure of PGC isolation of Gaok chicken with centrifugation gradient was using nycodenz as a substance. Commercially available cryoprotectants (dimethyl sulfoxide = DMSO) were used for PGC freezing. Isolated and frozen PGCs of Gaok chicken were diluted with cryoprotectants containing 2.5; 5 and 10% DMSO in fetal bovine serum (FBS). The recovery rate of 2.5; 5 and 10% DMSO concentration were 36.4; 48.2 and 48 % respectively. The viability of PGC after freezing was significantly higher for 5% DMSO compared with 2.5% DMSO (P&lt;0.05), but not different from 10% DMSO. It can be concluded that the concentration DMSO of 5 % was effectve contration in freezing Gaok chicken PGC.</p>
Highlights
It is reported by the International Union for Conservation of Nature (IUCN 2015) that more than 13% of bird species were endangered
Freezing primordial germ cell (PGC) of commercial chicken in a medium consisting of 10% serum and 5-10% dimethyl sulfoxide (DMSO) had been reported to have various recovery rates (RR) and viabilities of PGC
The success of a method in cryopreservation process might be determined through microscopic evaluation of PGC morphology
Summary
It is reported by the International Union for Conservation of Nature (IUCN 2015) that more than 13% of bird species were endangered. Recent adopted strategies to conserve the bird biodiversity both in situ and ex situ were not effective (Sawicka et al 2015). With the PGC cryopreservation as the genetic material seems effective for the conservation strategy. Freezing PGC of commercial chicken in a medium consisting of 10% serum and 5-10% DMSO had been reported to have various recovery rates (RR) and viabilities of PGC. Setioko et al (2007) reported that percentage of RR and viability with DMSO addition by 10% on WL chicken PGC each was 49.9% and 83.5%, respectively. Nakamura et al (2011) reported that addition of the same concentration of DMSO on Barred Plymouth Rock (BPR) showed recovery rate and viability by 54.3% and 86.8%, respectively. This study was expected to obtain optimal concentration of DMSO for RR and viability of Gaok chicken PGC after freezing and further feasible to be transferred
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