Abstract
The genus Pistacia L. belongs to the Anacardiaceae family and includes at least eleven species. Cytogenetic studies addressing the genus Pistacia are rather few. Chromosome numbers of the different Pistacia species, revealed by these studies, are questionable due to the fact that poor chromosome counting protocols were used, and these protocols are hampered by the extremely small-sized chromosomes of Pistacia species. The aim of this study was to develop a more effective method to resolve the chromosome numbers in Pistacia species using a fluorescent microscope. The method described here is modified from the Sigma Plant Protoplast Digest/Wash Solution protocol. The method used here is highly effective for karyotyping analysis and studying population genetics of Pistacia species. Moreover, it is easy and can be reproduced for other species that have smaller chromosomes. This method can be used for plant herbarium specimens or field plants. This study provides valuable chromosomal data for cytogeneticists and plant breeders who are working on this genus. It provides additional insight into understanding the taxonomic and phylogenetic relationships among Pistacia species. The chromosomes described here are also suitable for gene and genome mapping.
Highlights
The method described here is modified from the Sigma Plant Protoplast Digest/ Wash Solution protocol
The results show that all used Pistacia species are diploid and they have the same chromosome number of 2n = 30 (Figure 1)
The method was developed for Pistacia species because root-tips from the field are not possible to obtain and roots from seedlings are far too small
Summary
(2014) Effective Method to Resolve the Chromosome Numbers in Pistacia Species (Anacardiaceae). Previous studies showed that all Pistacia species are diploid with chromosome numbers 2n = 24, 28, and 30. A recent study by Ila et al [10] reported the chromosome number for the first time as 2n = 30 for the same species. Chromosome number of P. eurycarpa was reported as 2n = 30 [10] for the first time.
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