Effect on Insulin Output and Pancreatic Blood Flow of Glucagon, Secretin, Tolbutamide and Thiocid Infusions into an In Situ Isolated Portion of Pancreas

Abstract

Effects on blood flow and insulin output of the infusion of glucagon, secretin, tolbutamide and thiocid were studied on an isolated portion of dog pancreas with only one afferent and one efferent blood vessel remaining. Glucagon infused at the rate of 1 µg per minute initially gave a significant increase in pancreatic blood flow and insulin output. Infusion of glucagon at the rate of 10 µg per minute gave a significant increase in pancreatic blood flow and insulin output initially and throughout the infusion period. Secretin infused at the rate of 0.2 Unit per minute initially gave a significant increase in pancreatic blood flow and no significant change in insulin output. Infusion of secretin at the rate of 1.0 Unit per minute gave a significant increase in pancreatic blood flow and a highly significant increase in insulin output initially and throughout the infusion period. Tolbutamide infused at the rates of 0.6 and 3.0 mg per minute gave no change in pancreatic blood flow but increased the insulin output significantly. Thiocid (the preservative for tolbutamide) infused at pH 7.0, 8.5 and 9 gave no change in pancreatic blood flow or insulin secretion.