Abstract
Cultured porcine neutrophils were incubated in the presence of eitherActinobacillus pleuropneumoniae hemolysin protein (HP) in cell-free culture supernatant fluid or highly purified lipopolysaccharide (LPS) for times ranging from 2 to 15 min, followed by fixation and embedding for electron microscopy. Compared with untreated controls, neutrophils incubated in the presence of HP showed a progressive degeneration as evidenced by swelling, loss of cytoplasmic extensions, cytoplasmic vacuolation, and clumping of granules. Nuclear changes included the appearance of perinuclear spaces, nuclear membrane discontinuities, and disappearance of heterochromatin. These degenerative changes were cumulative and complete by 8 min of incubation time. In contrast, neutrophils incubated in LPS were unaffected compared with controls. Changes observed in neutrophils exposed to HP were similar to observed progressive changes in alveolar neutrophils from pigs exposed to aerosols ofA. pleuropneumoniae.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
