Abstract
Abstract The effects of different concentrations of Zataria multiflora Boiss. essential oil (EO: 0, 5, 15 and 30 μl 100 ml −1 ) and nisin ( N : 0, 0.25 and 0.5 μg ml −1 ), temperatures ( T : 25 and 8 °C), and storage times (up to 21 days) on growth of Salmonella typhimurium and Staphylococcus aureus in a commercial barley soup were evaluated in a factorial design study. The growth of S. typhimurium was significantly ( P N concentrations at 8 °C. For S. aureus , the viable count was significantly ( P N concentrations and their combinations, incubated at both storage temperatures. The mechanism of the antimicrobial action of EO, N , and their combinations against cell membranes of the tested organisms were also studied by measurement of the release of cell constituents and by the electronic microscopy observations of the cells. The significant increase of the cell constituents’ release of both organisms was observed as a result of treatments with EO and EO in combination with N . Electronic microscopy observations revealed that the cell membranes of S. typhimurium treated by EO and EO in combination with N were significantly damaged, while cells treated with only N looked similar to untreated cells. The electron micrographs of treated cells of S. aureus with EO, N , and their combination also showed important morphological damages and disrupted membranes.
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