Abstract
Objective To examine the effect of WNT2B on chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs). Methods Chondrocyte differentiation was induced following lentiviral-mediated overexpression of WNT2B in hBMSCs from one subject. Changes in expression of chondrocyte marker genes sex determining region Y box 9 (Sox-9) and Collagen Ⅱ were measured by quantitative polymerase chain reaction (qPCR). The number of Alcian blue-positive cartilage nodules, staining intensity of sulfated glycosaminoglycans secreted by hBMSCs-derived chondrocytes, and number of hypertrophic chondrocytes were determined by microscopy. Results WNT2B overexpression during chondrogenic differentiation of hBMSCs has no effect on Sox-9 gene expression (P=0.564), but significantly inhibited Collagen Ⅱ gene expression (P=0.009). Additionally, WNT2B significantly decreased the number of cartilage nodules (78.833±8.796 vs. 32.333±4.844, P=0.000), significantly reduced the staining intensity of sulfated glycosaminoglycans secreted by hBMSCs-derived chondrocytes (80.247±4.806 vs. 53.165±4.723, P=0.000), and significantly reduced the number of hypertrophic chondrocytes induced by three-dimensional culture [(13.833±2.994) cells vs. (6.667±1.211) cells, P=0.000]. Conclusion WNT2B can inhibit chondrogenic differentiation of hBMSCs in vitro. Key words: WNT2B; Bone marrow mesenchymal stem cells; Chondrocytes; Differentiation; Gene overexpression
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