Abstract
Using perfusing solutions of various salts approximating in ionic concentrations those found by Griffith1 to be present in the blood of Astacus fluviatilis, an attempt was made to determine the effect of the Na, K, Ca, and Mg ion upon the intact heart of the Crayfish (Cambarus clarkii). For comparative purposes a control perfusing solution containing 1% NaCl+0.028%—KCl+0.024% CaCl2 was employed. Such a solution applied to the heart maintained the normal rhythm for a period of several hours when perfused at a temperature of 25±0.1 degrees centigrade. Graphic records were obtained of the heart rhythm while being perfused with the various solutions.In determining the effect of the Na ion, 3 perfusing solutions were employed, viz.: A pure solution of 1% NaCl, one containing 2% NaCl+0.028% KCl+0.024% CaCl2 and one containing 0.028% KCl+0.024% CaCl2. Both solutions containing Na produced a marked increase in rate and amplitude of the beats coupled with a loss in tone. The pure 1% solution had an especially mark...
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