Abstract

This study was undertaken to investigate the effect of temperature (25 °C and 30 °C) and nitrogen supplementation (with 20 g/L peptone or without) on bioethanol production from waste bread, watermelon and muskmelon feedstocks by Saccharomyces cerevisiae. Nitrogen addition resulted in higher ethanol yields at both temperatures. Fermentation at 30 °C produced a higher ethanol yield than at 25 °C. The highest ethanol yield of 0.502 gethanol /g fermentable sugar, at 98.22% of the theoretical maximum, was obtained from the fermentation of muskmelon juice at 30 °C with nitrogen supplementation. Ethanol yields at both temperatures were significantly different (p < 0.05) between the trials with nitrogen and without nitrogen supplementation for watermelon and muskmelon but not for waste bread hydrolysate. On the other hand, ethanol yields showed significant differences (p < 0.05) at 25 °C and 30 °C for all feedstocks with nitrogen and without supplementation.

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