Effect of temperature and dissolved oxygen on biodegradation of nitrilotriacetate

Abstract

The effect of temperature and dissolved oxygen on the rate of biodegradation of nitrilotriacetate (NTA) was examined in water samples collected from the Rur River. Biodegradation of NTA was first order with respect to NTA concentration over a concentration range of 50–1000 μg l −1. First order rate constants showed a typical temperature dependency (temperature coefficient, Q 10 = 2) and biodegradation of NTA was observed over a temperature range of 2–24°C. The effect of temperature on the rate of NTA biodegradation was described by the Arrhenius equation, with calculated activation energies in the range reported for ordinary enzyme reactions. Biodegradation of NTA was also observed at low dissolved oxygen concentrations (0.3 mg l −1), although at reduced rates compared to high oxygen concentrations (13 mg l −1). Biodegradation of NTA was oxygen-dependent, suggesting an obligate oxygen requirement for the initial steps in NTA metabolism by natural microbial communities in surface waters. In general, our results indicate that NTA biodegradation will occur in natural waters under conditions of low temperature and low dissolved oxygen and also at low NTA concentrations.