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Effect of Sugary Drinks and Denture Cleanser on Color Stability and Surface Roughness of 3D Printed and CAD–CAM Milled Denture Tooth Materials. An In Vitro Study

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ABSTRACTObjective:This study aimed to evaluate the effect of various sugary drinks and a denture cleanser on the color stability and surface roughness of two tested denture teeth materials (3D printed and CAD/CAM milled).Materials and Methods:CAD/CAM additive and subtractive techniques were used to fabricate 160 custom disc-shaped specimens from two commercially available denture teeth resins. Specimens were randomly divided into 16 groups (n = 10) based on the immersion media used: a control group (artificial salivary substitute), sugary drinks groups (Pepsi, Gatorade, and ice tea), and denture cleanser groups after exposure to sugary drinks. These immersion cycles were repeated for 180 days, and the changes in color and surface roughness measurements were recorded. The data related to change in color and surface roughness was tabulated, and the statistical analysis was performed.Results:When exposed to sugary drinks, the change in color for both milled and 3D-printed materials was maximum when tested specimens were exposed to ice tea (ΔE = 3.548 and 4.055), followed by a carbonated drink (Pepsi) (ΔE = 2.334 and 3.503) and sports drink (Gatorade) (ΔE = 1.272 and 1.443), respectively. Whereas the change in surface roughness was highest after exposure to carbonated drink (ΔRa = 0.052 μm and 0.074 μm), followed by ice tea (ΔRa = 0.043 μm and 0.061 μm) and sports drink (ΔRa = 0.039 μm and 0.049 μm) for milled and 3D-printed materials, respectively.Conclusion:The 3D-printed denture tooth resins have poor color stability and are prone to more changes in surface roughness when exposed to different sugary drinks as compared to CAD/CAM milled denture teeth.

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  • Research Article
  • Cite Count Icon 102
  • 10.4103/0972-4052.197940
Effect of denture cleansers on color stability, surface roughness, and hardness of different denture base resins
  • Jan 1, 2017
  • The Journal of the Indian Prosthodontic Society
  • Anand Porwal + 3 more

Aim:The purpose of this study was to evaluate the effect of different denture cleansers on the color stability, surface hardness, and roughness of different denture base resins.Materials and Methods:Three denture base resin materials (conventional heat cure resin, high impact resin, and polyamide denture base resin) were immersed for 180 days in commercially available two denture cleansers (sodium perborate and sodium hypochlorite). Color, surface roughness, and hardness were measured for each sample before and after immersion procedure.Statistical Analysis:One-way analysis of variance and Tukey's post hoc honestly significant difference test were used to evaluate color, surface roughness, and hardness data before and after immersion in denture cleanser (α =0.05).Results:All denture base resins tested exhibited a change in color, surface roughness, and hardness to some degree in both denture cleansers. Polyamides resin immersed in sodium perborate showed a maximum change in color after immersion for 180 days. Conventional heat cure resin immersed in sodium hypochlorite showed a maximum change in surface roughness and conventional heat cure immersed in sodium perborate showed a maximum change in hardness.Conclusion:Color changes of all denture base resins were within the clinically accepted range for color difference. Surface roughness change of conventional heat cure resin was not within the clinically accepted range of surface roughness. The choice of denture cleanser for different denture base resins should be based on the chemistry of resin and cleanser, denture cleanser concentration, and duration of immersion.

  • Research Article
  • Cite Count Icon 10
  • 10.1111/jopr.13525
Effect of Effervescent Denture Cleansers on 3D Surface Roughness of Conventional Heat Polymerized, Subtractively, and Additively Manufactured Denture Base Resins: An In Vitro Study.
  • May 2, 2022
  • Journal of Prosthodontics
  • Saurabh Jain + 1 more

To compare the change in surface roughness of denture bases fabricated using three different techniques (additive manufacturing, subtractive manufacturing, and conventional heat-polymerizing) when immersed in two commonly available denture cleansers. One hundred and seventeen disc-shaped denture base specimens (39/group), were fabricated by subtractive manufacturing (Wieland), additive manufacturing (NextDent Denture 3D+), and conventional heat-polymerizing (Meliodent) techniques, following the manufacturers' instructions. Specimens were randomly divided into 3 groups and immersed in two effervescent denture cleansing solutions and distilled water to simulate 180 days of denture cleansing. A 3D optical noncontact surface profilometer was used to record the surface roughness of the tested denture base materials before and after immersion. Two-way ANOVA, followed by Bonferroni post hoc test, was used to assess the effects of denture cleansers on surface roughness of tested denture base resins. When immersed in Fixodent and Fittydent effervescent denture cleansing solutions, the highest change in absolute surface roughness (∆Sa, in μm) was observed in additively manufactured denture base material (0.181 ±0.018 and 0.079 ±0.008), followed by heat-polymerized denture base material (0.149 ±0.012 and 0.059 ± 0.011), while subtractively manufactured denture base material showed the least change (0.110 ±0.026 and 0.038 ±0.007), respectively. There was a difference in the extent of change in surface roughness between the denture cleansers. The change in surface roughness was much higher with the Fixodent denture cleanser as compared to the Fittydent denture cleanser. Subtractively manufactured denture base resin displayed the lowest change while additively manufactured denture base resin displayed the highest change in surface roughness in both denture cleansers, but the extent of change in surface roughness was variable.

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  • Cite Count Icon 5
  • 10.1016/j.prosdent.2023.09.008
Longevity of extrinsic stains on monolithic zirconia restorations: An in vitro study
  • Oct 14, 2023
  • The Journal of Prosthetic Dentistry
  • Shahed Bayestehtarat + 4 more

Longevity of extrinsic stains on monolithic zirconia restorations: An in vitro study

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  • Research Article
  • Cite Count Icon 22
  • 10.3390/biomedicines11020491
Effects of Smokeless Tobacco on Color Stability and Surface Roughness of 3D-Printed, CAD/CAM-Milled, and Conventional Denture Base Materials: An In Vitro Study
  • Feb 8, 2023
  • Biomedicines
  • Maryam H Mugri + 14 more

Tobacco consumption in its different forms can affect the optical and surface properties of dental materials that are used in the oral cavity. Thus, the present study was conducted to evaluate the effects of two commercially available smokeless tobacco products on the color stability and surface roughness of denture base resins that were fabricated using three different techniques (CAD/CAM milling, 3D printing, and conventional heat polymerization). A total of 126 denture base resin specimens were fabricated using the three different manufacturing techniques (n = 42 each). Specimens from each group were further subdivided into three subgroups (n = 14 each) and immersed in three different immersion media (a khaini suspension, a tabbaq suspension, and artificial saliva). The differences in color and surface roughness were assessed according to data that were collected and statistically analyzed using SPSS version 24.0. The tabbaq smokeless tobacco was found to cause greatest changes in color and surface roughness; the effect was observed to be highest in the 3D-printed specimens followed by the conventional heat-polymerized and CAD/CAM milled specimens. The mean changes in color and surface roughness were the highest for the tabbaq smokeless tobacco followed by the khaini smokeless tobacco and the artificial saliva. Statistically significant (p-value < 0.05) differences were observed among all techniques and suspensions. We concluded that the mean changes in color and surface roughness were significantly higher for the 3D-printed dentures compared to the conventional heat-polymerized and CAD/CAM-milled dentures. Thus, the results of the present study strengthened the concept that tobacco in any form can lead to changes in the color and surface roughness of denture base materials.

  • Research Article
  • Cite Count Icon 6
  • 10.3233/thc-230723
Effect of smokeless tobacco on color stability and surface roughness of CAD/CAM milled, 3D printed, and conventional provisional crown and fixed dental prosthesis materials: An in vitro study.
  • May 1, 2024
  • Technology and health care : official journal of the European Society for Engineering and Medicine
  • Mohammed E Sayed + 12 more

Provisional fixed dental prosthesis (FDP) plays an important role during fixed prosthodontic therapy till the definitive. Discoloration of materials used for provisional FDPs can cause patient dissatisfaction and may create doubt about the color stability of the definitive FDP. Surface roughness is the other major property to be taken into consideration for provisional FDP materials. Smokeless tobacco is reported to affect the color stability and surface roughness of different prosthetic materials. The aim of the current study was to evaluate the effect of two types of smokeless tobacco (black and white) on color stability and surface roughness of 3D printed, CAD/CAM milled, and conventional provisional FDP resin materials. A total of 144 disc-shaped specimens were fabricated using four techniques CAD/CAM subtractive technique (milling), CAD/CAM additive technique (3D Printing), and conventional technique using autopolymerized PMMA, and autopolymerized Bis-acrylic resins. Each group was subdivided into three subgroups of twelve specimens each, and were submerged into three solutions (artificial salivary substitute, black smokeless tobacco, white smokeless tobacco). The change in color and surface roughness was evaluated and the data collected were statistically analyzed. It was observed that black smokeless tobacco caused the maximum color change and the effect was highest in autopolymerized PMMA resin specimens (ΔE = 9.343 ± 0.489), followed by 3D printed (ΔE = 7.187 ± 0.391), autopolymerized Bis-acryl (ΔE = 6.464 ± 0.453) and milled (ΔE = 4.978 ± 0.227). White smokeless tobacco was found to cause a maximum change in surface roughness and the effect was highest in autopolymerized Bis-acryl specimens (ΔRa = 0.321 ± 0.015 μm), followed by autopolymerized PMMA (ΔRa = 0.297± 0.015 μm), 3D printed (ΔRa = 0.191 ± 0.019 μm), and milled (ΔRa = 0.168 ± 0.014 μm). Statistically significant (p-value < 0.05) differences were observed among all techniques and solutions. The change in color and surface roughness were maximum in the case of FDPs prepared using autopolymerizing resins, followed by 3D printed, and CAD/CAM milled reins.

  • Research Article
  • Cite Count Icon 1
  • 10.7759/cureus.74505
An In Vitro Study to Assess the Effect of Cigarette Smoke on Color Stability and Surface Roughness of 3D Printed, Milled, and Traditional Provisional Crown and Bridge Materials.
  • Nov 26, 2024
  • Cureus
  • Saurabh Jain + 9 more

Objectives To assess the influence of cigarette smoke (CS) on the color and surface roughness of 3D printed, milled, and traditionally fabricated provisional crown and bridge (PC&B) materials. Materials and methods 112 disc-shaped samples were made employing four techniques and materials (28 per group) to fabricate PC&B prostheses. Specimens were fabricated using standard protocols, such as 3D printing, milling, conventional bis-acrylic resin, and traditional autopolymerizing polymethyl methacrylate (PMMA) resin. After preliminary color and surface roughness recording, each group specimen was divided randomly into two subgroups (14 each). The artificial saliva acted as the storage media for the control group specimens for 30 days, and test group specimens were subjected to CS in a customized smoking chamber (10 minutes twice daily, for 30 minutes). Final color and surface roughness measurements were made. The change in color (∆E00) and surface roughness (∆Sa) were calculated, and the data was tabulated for analysis. Statistical analysis One-way analysis of variance was used to analyze the change in color and surface roughness. Post-hoc Tukey HSD test was used for comparison between groups. Results The mean ΔE00 and ∆Sa were higher among groups exposed to CS than those exposed to artificial saliva. The maximum change in color was recorded in the autopolymerizing PMMA, whereas the 3D printed resins recorded the minimal change.The traditional bis-acrylic resin recorded the maximum mean surface roughness change, while the milled resin recorded the least change. Conclusions Within the study limitations, it can be inferred that when exposed to CS, 3D printed and milled PC&B materials have superior color stability and displayed less change in surface roughness when equated with traditional bis-acrylic and autopolymerizing PMMA resins.

  • Research Article
  • 10.4103/jcrt.jcrt_945_22
A comparative evaluation of effect of denture cleansers on color stability, surface roughness and hardness of polyamide denture base material.
  • Apr 27, 2023
  • Journal of Cancer Research and Therapeutics
  • Vikas Punia + 4 more

Different denture cleansers have an effect on color and different surface properties of polyamide denture base material. Thus, selecting a denture cleanser solution for polyamide denture base materials should be based on the properties of the denture cleanser itself. The present in vitro study is aimed to evaluate and compare the effect of different denture base cleansers on color, surface roughness and hardness of polyamide denture base material. Thirty specimens of commercially available polyamide denture base material were fabricated and divided into three groups of 10 each. Specimens from each group were immersed in different denture cleansers (sodium perborate, thyme oil and ozonated water) for 10 mins per day for 180 days. Initial and final color, surface roughness and surface hardness of each specimen were measured and the difference was calculated. Data was tabulated and subjected to statistical analysis using One way ANOVA for pair wise comparison. Polyamide denture base resin exhibited some degree of change in color, surface roughness, and surface hardness in all denture cleansers but was not statistically significant. The choice of denture cleanser for different denture base resins should be based on the chemistry of resin and the cleanser, denture cleanser concentration and duration of immersion.

  • Research Article
  • Cite Count Icon 5
  • 10.1111/jopr.13944
The impact of Streptococcus mutans biofilms on the color stability and topographical features of three lithium disilicate ceramics.
  • Aug 31, 2024
  • Journal of prosthodontics : official journal of the American College of Prosthodontists
  • Abdulrahman A Balhaddad + 7 more

Secondary caries around ceramic restorations is the most common reason for the replacement of fixed dental prostheses (FDPs). Therefore, it is important to examine the susceptibility of different ceramic materials to biofilm formation. This study aimed to evaluate biofilm development and associated roughness and color alterations in three lithium disilicate ceramics: Emax CAD (EC), Emax Press (EP), and LiSi Press (LP). Streptococcus mutans biofilms were grown on the three ceramics (n = 10 per group) for 7 days. Surface roughness values and color alteration were assessed before and after the biofilm using a non-contact profilometer and spectrophotometer, respectively. Biofilm growth was evaluated using colony-forming units (CFUs) and scanning electron microscope (SEM) images. The data were analyzed using one-way ANOVA and Tukey tests. There was a significant (p≤0.001) growth of S. mutans colonies on EC (6.75 ± 0.56) and EP (6.72 ± 0.54) specimens compared to LP, which showed no biofilm growth. The change in average surface roughness (∆Ra, nm) was significantly lower (p < 0.001) in the EC specimens (0.029 ± 0.003) compared to the EP (0.055 ± 0.012) and LP (0.041 ± 0.010). When the changes in the Rv and Rt values were investigated, no significant difference was observed among the groups. Following the biofilm challenge, the change in color (∆E00) was significantly lower (p = 0.005) in the LP group (1.68 ± 1.45) compared to the EC group (3.89 ± 1.50) and no significant difference was observed between the EP group (2.74±1.01) and the other two ceramics (p ≥ 0.05). LP ceramics exhibited superior resistance to S. mutans biofilm formation and associated changes in surface roughness and color compared to the Emax CAD and Emax Press ceramics. These findings suggest that the LiSi Press material may be more favorable to mitigate the risk of secondary caries.

  • Research Article
  • Cite Count Icon 31
  • 10.1111/jopr.13429
Optical Properties and Color Stability of Denture Teeth-A Systematic Review.
  • Sep 25, 2021
  • Journal of prosthodontics : official journal of the American College of Prosthodontists
  • Mei Ting Tieh + 2 more

To systematically review past studies to determine the effect of various solutions on the color of denture teeth, thus answering the question in regards to which type of denture teeth has the best optical properties after exposure to various solutions. The method of measuring the color of artificial teeth was also evaluated as a secondary outcome. A search of studies that quantitatively investigated the influence of immersion solutions on the color change of denture teeth was conducted. Ovid MEDLINE, PubMed and Scopus databases were searched from 1997 to April 2021. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were used during article selection. Data regarding the effect of immersion solutions, accelerated aging and surface treatments on color change were gathered. Methodologies used to assess optical properties were also summarized and compared. The modified CONSORT checklist was used to determine the risk of bias of past studied included in this review. One hundred thirty-three studies were identified after removing duplicates. Forty-one studies were selected for full-text analysis, and 35 remaining papers met the inclusion criteria and were therefore included in this systematic review. Thirty-two in vitro studies and 3 in vivo studies were included in the review. All studies reported that immersion in various solutions has a significant influence on the change in color and optical properties of denture teeth. However, the discoloration of denture teeth is still clinically acceptable in most studies. Exposure to various solutions also affected the translucency parameter of denture teeth. Most studies also investigated the surface roughness and hardness along with the optical properties, and reported that immersion cycles did not cause changes in surface roughness of denture teeth, while hardness was affected. The optical properties of PMMA denture teeth have been studied extensively, whereas that of CAD/CAM and 3D printed denture teeth is limited. Color stability of CAD/CAM milled denture teeth is comparable to conventional PMMA denture teeth. There are contradictory findings in terms of color stability of 3D printed denture teeth as compared to conventional PMMA denture teeth. Staining by coffee is worst among the common beverages and solutions investigated. Denture teeth can show color changes after immersion in staining beverages as early as one week. The degree of discoloration of denture teeth after immersion is time dependent, with the larger extent in the initial phase.

  • Research Article
  • 10.1186/s12903-026-08404-5
Effect of staining beverages on color stability and surface roughness of additively manufactured permanent resin materials.
  • May 11, 2026
  • BMC oral health
  • Özge Unlu + 2 more

To evaluate the effects of commonly consumed staining beverages on the color stability and surface roughness of two additively manufactured permanent resin restorative materials. Sixty disk-shaped specimens (10mm × 2mm) were fabricated from two additively manufactured resin materials (Saremco Print Crowntec and VarseoSmile Crown Plus). Specimens were finished with standardized polishing procedures and color values (CIE L*a*b*) and baseline surface roughness (Ra) were recorded. Samples were randomly assigned to three immersion media (tea, coffee, distilled water; n = 10 per subgroup) and stored at 37°C for 14 days with daily solution renewal. Color measurements were repeated after 24h and 14 days, and color differences were calculated using the CIEDE2000 formula (ΔE₀₀). Surface roughness was re-measured after 14 days. Data were analyzed using mixed-model ANOVA and correlation tests (α = 0.05). Staining solution significantly affected color change at both evaluation periods (p < 0.001), while material type had no significant influence (p > 0.05). Tea produced the highest discoloration values, followed by coffee, whereas water groups remained below acceptability thresholds. Color change increased significantly over time in tea and coffee groups. Surface roughness showed only minimal changes after immersion, with Ra values ranging from 0.438 to 0.559μm at baseline and 0.444-0.567μm after 14 days, with no significant effects of material or staining solution (p > 0.05). No significant correlation was found between baseline surface roughness and final color change (p > 0.05). A significant positive correlation was observed between the change in surface roughness (ΔRa) and color change (ΔE00) (p < 0.05). Discoloration of additively manufactured permanent resin materials was primarily influenced by exposure to staining beverages. Tea and coffee caused clinically noticeable color changes, while surface roughness showed limited changes during short-term aging. However, a significant positive correlation between surface roughness change and color change suggests that surface degradation may contribute to staining susceptibility.

  • Research Article
  • Cite Count Icon 54
  • 10.1007/s00784-004-0275-2
Effect of tooth-whitening strips and films on changes in color and surface roughness of resin composites.
  • Jul 9, 2004
  • Clinical Oral Investigations
  • Ji-Hyung Kim + 4 more

Though the effectiveness of film and strip type tooth-whitening agents on the bleaching of tooth has been confirmed, there have been few studies on their influence on restorative materials. The purpose was to investigate the effect of tooth-whitening film and strip on the changes in color and surface roughness of dental resin composites. The composites used were Filtek Supreme (3M ESPE), Point 4 (Kerr), and Spectrum TPH (Dentsply). Specimens were prepared 10 mm in diameter and 1 mm in thickness. Film type (Night Effect, Crest; Simply White Night, Colgate) and strip type (Whitestrips Professional, Crest; Claren, LG) agents were used, and bleaching procedures were performed for 2 weeks according to the manufacturers' recommendations. Color was measured according to the CIELab color scale with a spectrophotometer. Color difference ( DeltaE(*)(ab)) and surface roughness ( Ra) were measured. After bleaching, the DeltaE(*)(ab) values were 0.90-1.67, which was too small to be perceptible (<3.3). The increase in Ra values was significant in some composite-whitening agent combinations ( P<0.01), however the value was less than 0.30 microm after bleaching. Therefore the change in surface roughness was clinically insignificant. We conclude that the influence of tooth-whitening film and strip on the color and surface roughness of dental resin composites was negligible.

  • Research Article
  • Cite Count Icon 123
  • 10.1046/j.1365-2842.2003.01014.x
Changes in surface roughness and colour stability of soft denture lining materials caused by denture cleansers.
  • Jan 17, 2003
  • Journal of Oral Rehabilitation
  • C Jin + 5 more

Soft denture lining materials were immersed into solutions of denture cleansers for 8 h at room temperature, and immersed into distilled water for the remainder of the 24-h period at 37 degrees C. Surface roughness of the soft denture lining materials was measured by contact type surface roughness instrument. For the colour stability test, soft denture lining materials were immersed in the denture cleansers as described above for 180 days. Finally, the colour changes of each material were quantitatively measured by a photometrical instrument to obtain the colour differences between newly processed specimen and immersed specimens (P < 0.01). An autopolymerizing silicone material, Evatouch, exhibited severe changes in surface roughness by all denture cleanser, and the generic material GC Denture Relining showed the minimal changes. Severe colour changes were also observed with some liner and cleanser combinations (P < 0.01). Except for Evatouth, the four silicone soft liners were more stable in surface roughness and in colour change than the two acrylic soft liners. One autopolymerizing silicone (GC denture relining) and one heat curing silicone (Molloplast B) demonstrated the best stability.

  • Research Article
  • 10.1016/j.prosdent.2015.01.020
Caries management by risk assessment (CAMBRA) and its effect on the surface roughness of various restorative materials
  • Jul 14, 2015
  • The Journal of Prosthetic Dentistry
  • Lauren M Bolding + 4 more

Caries management by risk assessment (CAMBRA) and its effect on the surface roughness of various restorative materials

  • Research Article
  • 10.4028/www.scientific.net/kem.867.125
Effect of Surface Treatment and Cutting Orientation to the Changes in Stents Surface Roughness
  • Oct 12, 2020
  • Key Engineering Materials
  • Achmad Syaifudin + 2 more

During the implantation process, an expandable balloon stent undergoes a change in mesh shape with a high strain rate. Permanent mesh shape changes to the stents indicate plastic deformation has occurred. On a micro-scale, plastic deformation has significant influence when interacting with the soft tissue of human blood vessels. This experimental study aims to investigate the effect of surface treatment and cutting orientation on the changes in surface roughness that definitely occurs when a stent deployed. To study the effect of surface treatment, two types of surface treatment were applied after surface polishing, i.e. etching and electropolishing. Surface polishing is carried out to enable microscopic observation. As for examining the effect of cutting orientation, the plate is cut in lateral and longitudinal orientation against the predicted-rolling direction of 316L sheet-type of stainless steel. An intermittent tensile test is conducted to obtain information about the changes in surface roughness. The surface observation is carried out three times on a similar surface of testpiece after reaching plastic deformation. The experimental study shows that the orientation of raw material has an insignificant effect on the changes in stent surface roughness. As for the surface treatments, electropolishing tended to decrease the tensile property of material.

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  • Research Article
  • Cite Count Icon 3
  • 10.20396/bjos.v17i0.8654158
Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMA
  • Dec 4, 2018
  • Brazilian Journal of Oral Sciences
  • Débora Oro Ferrari + 4 more

Denture use may aggravate the occurrence of oral infections, considering it enhances microbial adherence. Aim: This study assessed the reduction of microbial loads of Candida albicans, Staphylococcus aureus, and Klebsiella oxytoca by disinfecting the polymethylmethacrylate (PMMA) of complete dentures with hydroalcoholic extract of Salvia officinalis. Additionally, the effect of such extract on the properties of PMMA was examined. Methods: Microorganisms were isolated from saliva samples collected from complete denture wearers. The hydroalcoholic extract of S. officinalis was produced according to the Brazilian Pharmacopoeia 5. The PMMA specimens (n=188) were immersed in microbial inoculum and incubated at 37°C for 16 hours per day. Then, they were subjected to a disinfection protocol for 30 days. The specimens were divided into five treatment groups: sterile saline solution (0.85%; control), 0.2% chlorhexidine digluconate, and hydroalcoholic extract of S. officinalis (0.2%, 0.8%, and 1.16%). Microorganism adherence to the PMMA surface was also assessed, as well as surface roughness (Ra in µm) and color stability of the PMMA (mean ΔE). Changes in microbial load and surface roughness after the disinfection protocol were verified with paired t-test. Substances at day 10, adherence, and color stability were compared by the Kruskall-Wallis and Mann-Whitney tests, and one-way ANOVA was used to compare substances at the beginning and end of the experiment (α=0.05). Results: The 1.16% S. officinalis extract significantly reduced the microbial load of all the microorganisms after 30 days of disinfection (p&lt;0.05). The microbial load of K. oxytoca was also reduced at lower concentrations of the S. officinalis extract (0.2% and 0.8%) (p&lt;0.02). Antimicrobial and anti-adherent effects against microorganisms isolated from the oral cavity were observed. There was no significant change in surface roughness (p&gt;0.05) and color stability was significantly higher in the control group (p&lt;0.0001). Conclusions: The hydroalcoholic extract of S. officinalis may be used as a disinfectant solution for dentures.

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