EFFECT OF SUCCINATE-CONTAINING DRUGS ON CELLULAR COMPOSITION OF BLOOD IN RATS AT REST, DURING FREE SWIMMING AND WEIGHT-LOADED FORCED SWIMMING TEST

Abstract

The aim of the study is to analyze the effect of succinate-containing drugs on the cellular composition of blood in rats at rest, during free swimming and weight-loaded forced swimming test.
 Materials and Methods The study was carried out on practically healthy sexually mature male Wistar rats (250–300 g). Animals received a succinate-containing drug (meso-2,3-dimercaptosuccinic acid) at rest, before free swimming and weight-loaded swimming (4 % of body weight), and after 12 hours before exercise. Then, the levels of hemoglobin, hematocrit, the number of erythrocytes, reticulocytes and leukocytes with the distribution of their subpopulation composition were detected in the blood of rats. The authors measured the diameter of 100 reticulocytes and erythrocytes stained according to Romanovsky-Giemsa technique and by brilliant cresyl blue. The significance of differences in the reactions of rats’ blood cells was determined by the Kruskal-Wallis test using the R package WRS2(version 3.4.2).
 Results. The use of a succinate-containing drug in animals increased the proliferative activity foremost of lymphoid tissue. At rest, the indicators of the cellular composition of blood in rats corresponded to those after free swimming without succinate-containing drug use, which is considered as a transition of the functional state of the body to a level corresponding to the physical activity. The effect of the succinate-containing drug was more pronounced in animals during weight-loaded forced swimming test: duration of swimming increased by 2.8 times.
 Conclusion. In rats, the efficacy of a succinate-containing drug is manifested under intense physical activity. The practical use of a succinate-containing drug is physiologically justified if animals are under functional stress.