Abstract
The effect of six promoter-intron combinations on transient expression of β-glucuronidase (GUS) as the reporter gene was estimated in cultured einkorn ( Triticum monococcum), emmer ( Triticum durum) and common ( Triticum aestivum) wheat cell lines. Four promoters; cauliflower mosaic virus (CaMV) 35S, tandem CaMV35S, maize alcohol dehydrogenase gene ( Adh1) and rice actin gene ( Act1) promoters, and two introns; Adh1 intron1 and castor bean catalase intron, were tested. Six vectors having different promoter-intron combinations were introduced into wheat cells by using particle bombardment. Different levels of GUS gene activity in three wheat calli were detected by an in situ enzyme assay. The CaMV35S promoter gave the lowest level of transient expression in wheat cells. On the other hand, the rice Act1 promoter showed the highest level of transient expression in all three wheat cells and was also the most efficient promoter of all, suggesting that the rice Act1 promoter is efficient for use in wheat transformation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
