Effect of selenite and selenium nanoparticles on lactic bacteria: A multi-analytical study

Abstract

The effect of selenite and chitosan-modified SeNPs (CS-SeNPs) on Lactobacillus bulgaricus was evaluated through a multianalytical approach based on flow cytometry and transmission electron microscopy (TEM), and high performance liquid chromatography (HPLC) on line coupled to inductively coupled plasma mass spectrometry (ICP-MS) to investigate both lactic bacteria viability in presence of selenium compounds and transformation of selenium compounds once accumulated by the bacteria. For this purpose, L. bulgaricus were grown at 37°C for 24, 48 and 72h in presence of 1 and 10μg Se·mL−1 of selenium as CS-SeNPs and Na2SeO3. No significant differences in bacteria cell viability between selenium-enriched and control bacteria were observed when adding 1μg Se·mL−1 either as Se(IV) or CS-SeNPs. In contrast, bacteria viability decreases when increasing selenium concentration up to 10μg Se·mL−1 being this effect more accused when selenium was supplemented as selenite. Under these conditions SeNPs killed approximately 20% of Lactobacillus after 24h of exposure while the percentage increased up to 60% of bacteria when 10μg Se·mL−1 as selenite was applied. Transmission electron microscopy (TEM) images show that CS-SeNPs readily enter in the bacteria cells preserving membrane integrity and therefore causing no cellular damage. Results from HPLC-ICPMS indicate that most of the CS-SeNPs in the culture media were transformed by the bacteria to organo selenocompounds. A low transformation into organic forms was observed when L. bulgaricus were exposed to selenite, with Se(IV) being the major Se-specie identified. The fact that Lactobacillus was more efficient in transforming SeNPs than selenite could also explain the differences observed in viability.