Abstract
In recent decades, RNA binding motif (RBM) proteins have been widespread concerned by researchers. Among them, RBM5 is considered as a potential tumor suppressor gene in HCC. RBM10, also belonging to the RBM family, have similar structure and high homology with RBM5, indicating its potential as potential tumor suppressor genes. However, the role of RBM10 in tumors is controversial. The purpose of this study was to analyze the expression correlation and functional relationship of miR-21 and RBM10 in human hepatocellular carcinoma (HCC) tissues and corresponding tumor cells. Bioinformatics analysis showed that miR-21 and RBM10 were both highly expressed in HCC; similarly, the expression levels of miR-21 and RBM10 in HCC cells were significantly higher than those in normal hepatocytes. There was a positive correlation between miR-21 and RBM10. Furthermore, knockdown of RBM10 inhibited the proliferation, migration and invasion of SNU-398 cells, and simultaneous overexpression of miR-21 attenuated this inhibitory effect. Meanwhile, overexpressing RBM10 could promote the proliferation, migration and invasion of Hep G2 cells; while the stability of miR-21 could be reduced by knocking down RBM10. On the whole, the findings of this study indicate that RBM10 is involved in regulating the function and activity of human HCC cells by affecting the stability of miR-21. RBM10 cannot be simply summarized as a tumor suppressor or oncogene. Further studies are needed to clarify the role of RBM10 in tumors.
Highlights
The expression levels of RBM10 and miR-21 in hepatocellular carcinoma (HCC) tissues Online GEPIA was used to search for data related to HCC for analyzing RBM10 mRNA and miR-21 expressions
Corresponding results showed that the relative expression levels of RBM10 mRNA and miR-21 in HCC tissues were significantly higher than those in adjacent tissues or normal control tissues (Figs. 1A and 1B)
In the 10 pairs of HCC and adjacent tissues collected in our experiment, the results of quantitative PCR analysis were consistent with the results of data from database, which showed that RBM10 and miR-21 were abnormally high expressed in HCC tissues (Figs. 1C and 1D)
Summary
In many tumor tissues with elevated miR-21, there was no amplification of gene encoding miR-21. High expression of miR-21 in tumor tissues may not be associated with the amplification of genes, suggesting that miR-21 expression disorder may be explained by transcriptional or post-transcriptional regulation (Bertoli et al, 2015; Kumarswamy et al, 2011). It is still unclear with respect to the mechanism of the high abundance of miR-21 in tumor tissues so far. RBM5 is considered as a potential tumor suppressor gene in HCC, providing novel insight for early diagnosis, targeted therapy and chemotherapy resistance of HCC (Sutherland et al, 2010). On the basis of the role of RBM5, more researchers began to explore the relationship between other members of RBM family and tumors. With respect to the above, based on a cell model of HCC, the present study was carried out to reveal the abnormal expression of RBM10 and its relationship with miR21, so as to demonstrate the biological function of RBM10 in HCC cells innovatively
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