Abstract
In this study the effect of probiotic preparations and plant extract was investigated against eleven fish bacterial pathogens viz. Aeromonas hydrophila, Cellobiococcus sp., Enterobacter aerogenes, E. cloacae, Klebsiella pneumoniae, Salmonella sp., Shigella sp., Streptobacillus sp., Streptococcus sp., Pseudomonas fluorescens and Staphylococcus aureus. All these pathogens were found to be resistant for antibiotics viz. nitrofurantoin, amoxycillin, bacitracin, cephalothin, erythromycin, novobiocin, vancomycin , amphicillin, oxacillin and colistin. In vitro antagonism test of the probiotics was performed by using well diffusion method. In case of probiotic AquaproTM, effective zone of inhibition of 2.433 cm was observed for P. fluorescens and K. pneumoniae. The extracts of Azadirachta indica, Aloe barbadensis, Withania somnifera and Momordica charantia were studied in vitro alone as well as in combination with probiotic (Lactobacillus sporogenes). The zone of inhibition observed in mixed sample was less as compared to individual sample. Among the four plants extract, the W. somnifera extract was found to be the most effective and it leads to maximum inhibition (1.1 cm) recorded for E. aerogenes.
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