Abstract
Polyethylene glycol (PEG) is often used to produce water stress in plant cell suspension cultures. The amount of soluble protein extracted from potato cell suspension cultures treated with PEG-6000 (10% w/v) decreased to 50%. In addition, the activities of glucose-6-P dehydrogenase, glutamate dehydrogenase and pyrroline-5-carboxylate reductase in these extracts were differentially reduced. High concentrations of PEG ( > 5%) in the enzyme assay systems were required to significantly affect the activities of the three enzymes extracted from PEG untreated cells. PEG must be removed from cells before proteins are extracted or enzymes assayed. A rapid procedure to harvest cells and subsequently to remove PEG from cells stressed with this compound is described.
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