Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.).

Abstract

This study examines the influence of the polyamines putrescine and spermidine on the efficiency of androgenesis in anther cultures of 2 carrot cultivars, Kazan F_1 and Narbonne F_1, and the effect of putrescine on the process of plant regeneration from androgenetic embryos. In the Kazan F_1 variety, an increase in the number of obtained embryos was achieved using each of the 2 polyamines separately and in combination. In contrast, no beneficial effects of polyamines on the efficiency of androgenesis were observed in the Narbonne F_1 variety. Putrescine added to the regeneration medium increased the number of obtained plants. For embryos obtained on induction medium without putrescine, the best concentration was 160 mg putrescine in 1 L of regeneration medium, and for those obtained on the induction medium with putrescine the best concentration was 0.5 mg putrescine per 1 L. All of the resulting plants, both in the experimental and control combinations, had a doubled set of chromosomes. The vast majority of them were homozygous for both isoenzymes, glucose-6-phosphate isomerase and aspartate aminotransferase, and the distribution of homo- and heterozygous received combinations with the polyamines and in the control was very similar.