Abstract
Callus was initiated from immature embryos of winter hexaploid triticale cultivars Bogo, Moniko, Moreno, Newcale, Salvo and Vero. Salvo was used in its original version (cytoplasm Triticum aestivum) and as alloplasmic (fertile) inbred line (B11) with Triticum timopheevi cytoplasm (SalvoTt). The MS induction medium was supplemented with 4 mg/cm3 of dicamba. Regeneration was performed on hormone free MS medium. In this study, the influence of genotype, embryo size and cold treatment during the induction phase on the culture efficiency were determined. Calli fresh weight, their differentiation pattern and plant regeneration rate were evaluated. On average from 3 to 14 plants per plated embryo were obtained. Genotype, embryo size and their interaction significantly influenced the culture parameters while the treatment of 5 °C (10 days) applied after 4 weeks of induction made no effect. Salvo and SalvoTt responded at the highest rate to the culture conditions, however T. timopheevi cytoplasm influenced negatively the regeneration rate. Embiyo at late globular or early scutellar stage produced calli with the highest regeneration potential.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
