Abstract
Summary The effect of the glutamate analogue phosphinothricin on enzymes of nitrogen assimilation was studied as well as the accumulation of ammonia and nitrate after treatment of lucerne plants with this herbicide. Glutamine synthetase (GS) was assayed with several phosphinothricin concentrations. When plants were sprayed with 250, 500 and 1,000 μM of the herbicide, GS activity of the leaf was reduced by 50% at 250 μM. Phosphinothricin caused a high ammonia accumulation in the tissues, reaching values of 70 μmol ammonia/g dry weight after treatment with 1,000 μM phosphinothricin. Glutamate dehydrogenase (GDH) activity increased ca. 40 % and nitrate reductase activity (NRase) was strongly decreased. Neither nitrate content nor proteolytic activity were altered.
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