Abstract
There are several substances that needs to be fulfill to keep the brain cell growth such as AA, DHA and EPA. Fungi is one of the alternative source of omega 3, omega 6, omega 9 especially AA, DHA and EPA. This research variates operating condition that is suitable for the growth of Aspergillus oryzae in AA, DHA, and EPA fatty acid production with Submerged Fermentation using synthetic medium. Aspergillus oryzae cultivated in medium using glucose as carbon source and Ammonium sulfate and yeast extract as nitrogen source. The extraction method using ethanol and n-hexane as solvent. The result shows that optimum agitation rate for unsaturated fatty acid production of Aspergillus oryzae is 120 RPM, lipid yield 28,28% and unsaturated fatty acid content 50,36 % and EPA content 2,42 %. Optimum medium pH for PUFA production of Aspergillus oryzae is 6, lipid yield 22,35 % and unsaturated fatty acid content 45,5 %. optimum incubation temperature for unsaturated fatty acid production of Aspergillus oryzae is 25 °C, lipid yield 13,19 % and unsaturated fatty acid content 62,15 %. Unsaturated fatty acids produced from Aspergillus oryzae are oleic, linoleic, linolenic and EPA.
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