Abstract
Conjugation of biological (peptides/enzymes) and synthetic polymers has potential applications in both medicine and biotechnology. We have shown in our previous article that the enzymatic activity of lysozyme was suppressed by the complex formation with a cationic smart copolymer, poly(N,N-diethylaminoethyl methacrylate)-graft-poly(ethylene glycol) (PEAMA-g-PEG). In addition, the secondary structure of lysozyme was maintained. In the present study, tertiary structure of lysozyme were investigated using circular dichrosm (CD) and fluorescence spectroscopy. Near-UV CD spectral analysis clearly indicates that the complexation of lysozyme with PEAMA-g-PEG has almost no influence on the tertiary structure of lysozyme. Moreover, fluorescence spectrum of lysozyme and lysozyme complexed with PEAMA-g-PEG indicates the conformations of lysozyme were not disrupted.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Bangladesh Journal of Veterinary and Animal Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
