Effect of Notoginsenoside R1 on autologous adipose graft in rats.

Abstract

Autologous fat particle transplantation has been widely used by surgeons. The present study evaluated the effect of Notoginsenoside R1 (NR1) treatment on rat autologous fat graft, along with the quality and retention rates. Male Sprague‑Dawley rats (n=60) received fat particle auto‑transplantation from the left abdominal cavity into lateral dorsum. A total of 14days after surgery, NR1 in different doses (50, 100 and 200mg/kg/day) was injected into rats, following which blood and fat graft samples were harvested at days 7, 14 and28. Assessments were carried out by hematoxylin and eosin staining, western blotting, ELISA and immunohistochemistry (IHC). The survival rate of fat grafts was increased in three experimental groups, as detected by weight measurement. Histological scoring demonstrated that there were significant differences in tissue integrity between the 100mg/kg/day group and the other 3 groups. hepatocyte growth factor, vascular endothelial growth factor, fibroblast growth factor, angiotensin and S100 levels in the 100mg/kg/day NR1 group was increased compared with the other 2 treatment groups; however, all 3 treatment groups demonstrated increased expression of these proteins compared with the control group. Additionally, cluster of differentiation (CD)68 exhibited negative expression and CD31 showed weakly positive expression in all three experiments, as assessed by IHC. In conclusion, 100mg/kg/day NR1 may potentially promote the retention rate and enhance the quality of autologous fat grafts via increasing vascularity in the recipient site. These results implicate NR1 as a therapeutic strategy for the improvement of outcome following fat graft surgery.